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Substantiivi
- (physics, microscopy) Fluorescence of a material when the source of stimulating radiation is behind the object, from the point of view of the observer.
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- For example, in contrast to transfluorescence microscopes, the intensity of illumination and consequently the amount of excitation energy increases as a function of increasing magnification.
- Likewise in fluorescence applications, an epifluorescence configuration is normally chosen over transfluorescence.
- Epifluorescence involves illuminating the specimen from above while in transfluorescence the excitation light comes from below the sample [Weeks, 2004].
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