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1. (chemistry) To separate (a mixture) into its individual constituents by exploiting differences in some chemical or physical property, such as boiling point, particle size, solubility etc.
2. (radiotherapy) To divide a total dose of radiation into fractions.
3. (cryptography) To divide each plaintext symbol into several ciphertext symbols as a preliminary stage of encryption.
4. To use the technique of fractionation in hypnosis.